Nestin (NES) Antikörper
| Antigen |
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| Synonyme |
FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (86), Maus (36), Ratte (Rattus) (33), Rind (Kuh) (7), Kaninchen (7), Hund (6), Pferd (4), Schwein (4), Hamster (2), Huhn (1), Schimpanse (1), Affe (1), Schaf (1)
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Applikation
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 Alternativen ELISA, Western Blot (WB), Immunhistochemie (IHC), Immunfluoreszenz (IF)
Western Blot (WB) (70), Immunfluoreszenz (IF) (60), Durchflusszytometrie (FACS) (50), Immunhistochemie (Paraffinschnitte) (IHC (p)) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (11), Enzyme Immunoassay (EIA) (10), Immunhistochemie (IHC) (10), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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| Produktnummer |
ABIN411540 |
| Menge |
100 µg (0.25 mg/ml) |
| Preis |
312,13 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human and mouse. Tested in ELISA, WB, IHC and IFC.
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Weitere Bezeichnung
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Nestin
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Gen-ID
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10763
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Immunogen
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Synthetic peptide selected from the N-terminal region of human Nestin
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Isotyp
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IgG (Passende Sekundärantikörper)
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Beschreibung
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Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, Nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
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Spezifität
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The antibody detects a ~ 177 kDa band, corresponding to the expected molecular mass of Nestin on immunoblots.
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Applikationshinweise
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The antibody can be used for ELISA (0.25 µg/ml), Western Blotting (2.5 – 5.0 µg/ml), Immunohistochemistry (2.5 – 5.0 µg/ml) and Immunofluorescence (5 - 25 µg/ml).
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Konzentration
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0.25 mg/ml
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Reinigung
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Affinity Purified Antibody
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Buffer
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100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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Lagerung
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Forschungsgebiet
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Angiogenese, Abstammungsmarker
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Nestin (NES)", Typ "Antikörper" finden
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Wirte
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Kaninchen (80), Maus (30), Huhn (3), Ziege (1)
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Reaktivitäten
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Human (86), Maus (36), Ratte (Rattus) (33), Rind (Kuh) (7), Kaninchen (7), Hund (6), Pferd (4), Schwein (4), Hamster (2), Huhn (1), Schimpanse (1), Affe (1), Schaf (1)
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Applikationen
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Western Blot (WB) (70), Immunfluoreszenz (IF) (60), Durchflusszytometrie (FACS) (50), Immunhistochemie (Paraffinschnitte) (IHC (p)) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (11), Enzyme Immunoassay (EIA) (10), Immunhistochemie (IHC) (10), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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Konjugate
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Biotin (4), HRP (4), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitope
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C-Term (4), AA 1484-1500 (1), AA 544-776 (1)
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