IL-18 Antikörper

Antigen: IL-18

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus

Applikation: Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN411516

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-murine IL-18 polyclonal antibody. Tested in Western blotting/Immunoprecipitation/Neutralization.

Gen-ID: 16173

Immunogen: Synthetic peptide of mouse IL-18

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: IL-18 (IFN-gamma-inducing factor, IGIF) is a novel proinflammatory cytokine that augments natural killer (NK) activity in spleen cells. It also has other biological properties such as activation of NF-kB, stimulation of Fas ligand expression, induction of CC and CXC chemokines. IL-18 increases production of competent HIV. IL-18 is related to the IL-1 family. One of the IL-18 receptor chains is the IL-1 receptor-related protein, a member of the IL-1R family. Like IL-1β, IL-18 is first synthesized as a leaderless precursor requiring the IL-1β converting enzyme (ICE) for cleavage into an active molecule. IL-18 may play a role in tissue injury in inflammatory reactions.

Spezifität: The antibody can be used for Western blot analysis (0.5-4 μ g/ml), immunoprecipitation (2-5 μ g/ml), and neutralization (2-5 μ g/ml). However, the optimal conditions should be determined individually.

Anwendungen

Applikationshinweise: Western blot analysis (0.5-4 µg/ml), immunoprecipitation (2-5 µg/ml), and neutralization (2-5 µg/ml). However, the optimal conditions should be determined individually.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-mouse IL-18 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Forschungsgebiet: Zytokine, Immunologie, Virologie, Inflammation, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: McCoy, Koizumi, Toma et al.: "Cytotoxins of the human pathogen Aeromonas hydrophila trigger, via the NLRP3 inflammasome, caspase-1 activation in macrophages." in: European journal of immunology, 2010 (PubMed).