GFP Antikörper

Antigen: GFP

Klonalität: Monoklonal (BV-F4)

Wirt: Maus

Applikation: Western Blot (WB)

Produktnummer: ABIN411495

Produktbeschreibung

Produktmerkmale: 100 ug antigen affinity purified mouse anti-GFP monoclonal antibody (IgG1). Recognizes wild-type GFP and its derivatives such as EGFP/EBFP/ECFP/EYFP, etc. Performs well in Western blot analysis, immunoprecipitation, and immunohistochemistry. However, the optimum conditions should be determined individually.

Gen-ID: 110612126

Immunogen: Recombinant EGFP expressed from E. col

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: BV-F4

Beschreibung: Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can also fuse with proteins of interest without interfering significantly with their assembly and function.

Spezifität: The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 μ g/ml), immunoprecipitation, and immunohistochemistry. However, the optimum conditions should be determined individually.. Recombinant RFP (Cat.# 4997-100) can be used as a positive control.

Anwendungen

Applikationshinweise: Western blotting (0.5-4 µg/ml), immunoprecipitation (2-8 µg/ml), immunofluorescence (10-20 µg/ml) and immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 µg/ml), immunoprecipitation, and immunohistochemistry. However, the optimum conditions should be determined individually.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) antigen affinity purified mouse anti-GFP monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Forschungsgebiet: Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar