Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) Antikörper

Antigen: Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3)

Synonyme: NOS, INOS, NOS2A, HEP-NOS, iNOS, Nos-2, Nos2a, i-NOS, NOS-II, eNOS, Nos-3, ecNOS, 2310065A03Rik, iNos, cNOS, EC-NOS, NOSIII, NOS3, NOS2, nos2, ECNOS

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN411480

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-eNOS polyclonal antibody. Reacts with human/mouse/rat/hamster/dog/cattle/sheep/guinea pig. Detects the 140 kDa full length and the ~100 kDa and 70 kDa eNOS fragments in Western blot analysis. Blockign peptide (Cat.# 3426RBP-50) is avaialble separately.

Weitere Bezeichnung: eNOS

Gen-ID: 24600

Immunogen: Synthetic peptide surrounding amino acid 602 of rat eNOS

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: ENOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation.

Spezifität: The antibody recognizes 140 kDa eNOS of human, mouse, and rat origins. Reactivity to other species has not been tested.

Anwendungen

Applikationshinweise: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 140 kDa eNOS of human, mouse, and rat origins. Reactivity to other species has not been tested.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Beschränkungen: Nur für Forschungszwecke einsetzbar