DR3/Apo3 Antikörper

Antigen: DR3/Apo3

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN411470

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-DR3 (also called Asl-1, Apo3, Apo-3, TRAMP, LARD, etc.) polyclonal antibody. Detects 48 kDa DR3 in Western blotting. Reacts with human/mouse/rat. Other species have not been determined. Blockign peptide (Cat.# 3057RBP-50) is available separately.

Gen-ID: 85030

Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 378 of mouse DR3

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Apoptosis can be induced by certain cytokines including TNF and Fas in the TNF superfamily through interaction with their death domain (DD) containing receptors, TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR3 (also called Wal-1, Apo3, TRAMP and LARD). The ligand for this novel death receptor has been defined as TWEAK (also termed Apo3L). Like TNFR1, overexpression of DR3 triggers apoptosis and NF-kB activation. Activation of DR3 results in recruitment of TRADD (a DD containing adapter molecule). TRADD associates with FADD, TRAF2, and RIP to activate the signaling complex for NF-kB activation. DR3 activates apoptosis through TRADD, FADD, and caspase-8. DR3 is highly expressed in the tissues enriched in lymphocytes including peripheral blood leucocytes (PBL), thymus and spleen.

Spezifität: The antibody detects a ~48 kDa DR3 in samples from human, mouse, and rat origins.

Anwendungen

Applikationshinweise: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) antigen affinity-purified rabbit anti-DR3/Apo3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Beschränkungen: Nur für Forschungszwecke einsetzbar