Dickkopf Homolog 2 (Xenopus Laevis) (DKK2) Antikörper

Antigen: Dickkopf Homolog 2 (Xenopus Laevis) (DKK2)

Synonyme: DKK-2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN411465

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-DKK2. Reacts with human/mouse/rat. Detects ~57 kDa Dkk2 in Western blot analysis. Blocking peptide is available separately.

Weitere Bezeichnung: DKK2

Gen-ID: 56811

Immunogen: Synthetic peptide surrounding amino acid 241 of mouse Dkk2

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Xenopus Dickkopf (Dkk)-1 was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of Dkk1 and Dkk2 inhibited Wnt-3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of Dkk-2, but not that of Dkk-1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.

Spezifität: The antibody recognizes 57 kDa Dkk2 in samples from human, mouse and rat origins. A ~55 kDa and ~28 kDa Dkk2 fragments can also be detected in rat kidney tissue lysate. Reactivity to other species has not been determined.

Anwendungen

Applikationshinweise: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 57 kDa Dkk2 in samples from human, mouse and rat origins. A ~55 kDa and ~28 kDa Dkk2 fragments can also be detected in rat kidney tissue lysate. Reactivity to other species has not been determined.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit Dkk2 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Forschungsgebiet: Stammzellen, Wnt

Beschränkungen: Nur für Forschungszwecke einsetzbar