Cathepsin D (CTSD) Antikörper

Antigen: Cathepsin D (CTSD)

Synonyme: CPSD, CLN10, MGC2311, CD, CatD, catD, fb93e11, fj17b09, wu:fb93e11, wu:fj17b09, CTSD, LOC398557, Cathd, LOC100136761, DKFZp469J0315

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN411447

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-Cathepsin D polyclonal antiboody. Detects the Procathepsin D (52 kDa) and also the active forms of cathepsin D (34 kDa). Reacts with human, mouse and rat. Tested in Western blot analysis. Blocking peptide (Cat.# 3191RBP-50) is available separately.

Weitere Bezeichnung: Cathepsin D

Gen-ID: 13033

Immunogen: Synthetic peptide surrounding amino acid 140 of mouse cathepsins D

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two-chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.

Anwendungen

Applikationshinweise: Western blot analysis (0.5-4 µg/ml) and in immunoprecipitation. However, the optimal conditions should be determined individually. The purified antibody detects both the 52 kDa proform and the 34 kDa cleaved fragment of Cathepsin D.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-cathepsin D polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Forschungsgebiet: Proteolyse / Ubiquitin, Extrazelluläre Matrix, Proteasen, Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar