Apolipoprotein E-II (apoe2) Antikörper

Antigen: Apolipoprotein E-II (apoe2)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN411426

Produktbeschreibung

Produktmerkmale: 100 ug affinity purified rabbit anti-human ApoE2 polyclonal antibody. Perform well in Western blot. Other applications have not been tested. Recombinant Human ApoE2 (Cat.# 4760-50) can be used as a positive control.

Weitere Bezeichnung: ApoE2

Gen-ID: 348

Immunogen: E. coli expressed recombinant human ApoE2

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. Compared with E3 and E4, E2 exhibits the lowest receptor binding affinity. E2 allele carriers had significantly lower levels of total cholesterol, low-density lipoprotein cholesterol, and non-high-density lipoprotein cholesterol, as well as increased ApoE levels. ApoE2 is a 34.3 kDa protein containing 300 amino acid residues.

Anwendungen

Applikationshinweise: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human ApoE2 (Cat. # 4760-50) can be used as a positive control.

Konzentration: 0.5 mg/ml

Reinigung: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti- human ApoE2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.

Lagerung: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Forschungsgebiet: Krebs, Artherosklerose, Metabolismus, Alzheimer-Krankheit (Morbus Alzheimer)

Beschränkungen: Nur für Forschungszwecke einsetzbar