Myc Tag Antikörper (HRP)

Details zu Produkt Nr. ABIN401754, Anbieter: Anmelden zum Anzeigen
Antigen
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • MYC proto-oncogene, bHLH transcription factor
  • MYC
Reaktivität
Tag
216
41
14
12
6
5
3
1
1
Wirt
Kaninchen
163
63
21
17
3
2
1
Klonalität
Polyklonal
Konjugat
HRP
27
24
15
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
226
121
82
53
52
39
23
19
18
7
6
5
5
5
5
4
3
3
2
2
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
Sequenz EQKLISEEDL
Spezifität This antibody is directed against human c-myc.
Produktmerkmale Absorption / Emission: 770 nm / 794 nm
Molar Ratio: 1,8 moles DyLight(TM) 800 per mole of Rabbit IgG
Reinigung Affinity chromatography
Andere Bezeichnung C-Myc Epitope Tag
Substanzklasse Tag
Hintergrund Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag
Gen-ID 4609
NCBI Accession NP_002458
UniProt P01106
Forschungsgebiet Transcription Factors, Cancer, Cell Cycle
Applikationshinweise ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/1,000 - 1/5,000.
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 0.1 mL of deionized water (or equivalent).
Konzentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as perservative
Konservierungsmittel Gentamicin sulfate
Vorsichtsmaßnahmen Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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