Myc Tag Antikörper (HRP)

Produktnummer ABIN401754

Produktdetails anti-Myc Tag Antikörper (HRP)

Target: Myc Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Myc Tag Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Sequenz: EQKLISEEDL

Spezifität: This antibody is directed against human c-myc.

Produktmerkmale: Absorption / Emission: 770 nm / 794 nm
Molar Ratio: 1,8 moles DyLight(TM) 800 per mole of Rabbit IgG

Aufreinigung: Affinity chromatography

Immunogen: Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/1,000 - 1/5,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water (or equivalent).

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as perservative

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Myc Tag

Target: Myc Tag

Andere Bezeichnung: C-Myc Epitope Tag (Myc Tag Produkte)

Substanzklasse: Tag

Hintergrund: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag

Gen-ID: 4609

NCBI Accession: NP_002458

UniProt: P01106