Pogz Antikörper

Antigen: Pogz

Synonyme: AU015913, AU044539, 9530006B08Rik

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Hund

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN401436

Produktbeschreibung

Produktmerkmale: Synonyms: Kiaa0461, Pogo transposable element with ZNF domain

Swiss-Prot: Q8BZH4

Immunogen: Synthetic peptide corresponding to a region near the N-terminus of mouse Pogz protein

Kreuzreaktivität: Hund, Schimpanse, Huhn, Human, Maus, Ratte (Rattus), Rind (Kuh), Anubis-Paviane, Affe

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Pogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromereprotein-B) domain, and a DDE domain found in the bacterial transposase/retroviralintegrase family. Members of this family of integrases share structural homologies andshow similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)Jrecombination in lymphoid progenitors. Mouse Pogz has a human homolog with a basepair similarity of 90% and amino acid similarity of 93% for the full length protein. Inhumans at least 3 variants have been predicted and they are all similar at the N-terminus. A number of expressed sequence tags (ESTAs) cloned from murine undifferentiated EScells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogzgene, further underlining an important function for this gene in stem cells. In mice,deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.

Spezifität: This antibody is known to react with Pogz protein. Species Reactivity: Tested: Mouse. Expected from sequence similarity: Human, Canine, Cattle, Rat, Chimpanzee, Macaque,Olive Baboon, Chicken, Short-tailed opossum.

Anwendungen

Applikationshinweise: ELISA: 1/150,000. Western Blot: 1/3,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.564 mg/ml (by UV absorbance at 280 nm)

Reinigung: Aff - Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Storage Conditions for Trial Size: This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volumedilute 1: 10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mixthoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use thisintermediate dilution when calculating final dilutions as recommended below. Shelf life: 3 month from despatch.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar