SMAD3 Antikörper (C-Term)

Produktnummer ABIN401333

Produktdetails anti-SMAD3 Antikörper

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Bindungsspezifität: AA 417-425, C-Term

Reaktivität: Human, Maus, Ratte, Schwein, Huhn, Zebrafisch (Danio rerio), Rind (Kuh), Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against Smad3 protein. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Human Smad3.

Kreuzreaktivität (Details): Species reactivity (expected):Xenopus laevis, Xenopus tropicalis, Zebrafish, Rat, Mouse, Porcine, Bovine, Chicken.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 417-425 of Human SMAD3 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.17 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: SMAD3 (SMAD3 Produkte)

Synonyme: HSPC193 antikoerper, HsT17436 antikoerper, JV15-2 antikoerper, LDS1C antikoerper, LDS3 antikoerper, MADH3 antikoerper, madh3 antikoerper, madh3a antikoerper, smad3 antikoerper, wu:fa99e03 antikoerper, XSmad3 antikoerper, XenMLP antikoerper, Xmad3 antikoerper, madh3-A antikoerper, Madh3 antikoerper, AU022421 antikoerper, Smad 3 antikoerper, mad3 antikoerper, madh3b antikoerper, zgc:92234 antikoerper, SMAD family member 3 antikoerper, SMAD family member 3a antikoerper, SMAD family member 3 L homeolog antikoerper, SMAD family member 3b antikoerper, SMAD3 antikoerper, smad3a antikoerper, smad3.L antikoerper, Smad3 antikoerper, smad3b antikoerper

Hintergrund: Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (RSmads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-β pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-beta-mediated response.Synonyms: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3

Gen-ID: 4088

NCBI Accession: NP_001138574

UniProt: P84022

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie