H2AFV Antikörper (pSer137)

Produktnummer ABIN401328

Produktdetails anti-H2AFV Antikörper

Target: H2AFV (H2A Histone Family, Member V (H2AFV))

Bindungsspezifität: AA 132-141, pSer137

Reaktivität: Drosophila melanogaster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser H2AFV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pSer137 residue. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Less than 0.2 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein.

Aufreinigung: Affinity Chromatography: The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.

Immunogen: Synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/4,000-1/16,000. Western Blot: 1/400-1/1,600.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at-20 °C. This product is stable for several weeks at 2-0 °C as an undiluted liquid. Dilute only prior to immediate use.

Details zu H2AFV

Target: H2AFV (H2A Histone Family, Member V (H2AFV))

Andere Bezeichnung: Histone H2A.v (H2AFV Produkte)

Synonyme: H2A.Z-2 antikoerper, H2afv antikoerper, H2AV antikoerper, h2av antikoerper, C530002L11Rik antikoerper, H2a.z-2 antikoerper, H2av antikoerper, H2A.F/Z antikoerper, h2afv antikoerper, h2afz antikoerper, H2A histone family, member V antikoerper, H2A histone family member V antikoerper, H2A histone family member V S homeolog antikoerper, H2A histone family, member Va antikoerper, H2afv antikoerper, LOC100226437 antikoerper, H2AFV antikoerper, h2afv.S antikoerper, h2afva antikoerper

Hintergrund: Variant histones H2A are synthesized throughout the cell cycle and are very different from classical Sphase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX "knock out" mice have an increased incidence of cancer.Synonyms: CG5499, H2A.F/Z, H2AFV, H2AV, H2AvD, His2Av, His2AvD

Gen-ID: 43229

NCBI Accession: NP_524519

UniProt: P08985