MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457)

Details for Product anti-FRAP1 Antibody No. ABIN401320, Anbieter: Anmelden zum Anzeigen
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Alternativen
anti-Human MTOR Antikörper für Immunofluorescence (fixed cells)
Epitop
AA 2440-2457
64
28
26
22
21
20
15
15
11
10
9
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Zebrafisch (Danio rerio)
368
200
199
18
8
8
7
7
7
7
4
2
2
1
1
1
Wirt
Kaninchen
316
48
3
1
Klonalität
Polyklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
8
8
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
246
111
94
73
55
52
37
20
7
7
6
6
3
2
2
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Immunogen Synthetic peptide corresponding to amino acids 2440-2457 of human mTOR
Isotyp IgG
Spezifität Reactivity occurs with both phosphorylated and non-phosphorylated forms of mTOR at S2448 from derived tissues and cells.
Kreuzreaktivität (Details) Species reactivity (expected):Rat, Mouse, Zebrafish, Canine.
Species reactivity (tested):Human.
Reinigung Immunoaffinity chromatography
Andere Bezeichnung mTOR / FRAP1 (FRAP1 Antibody Abstract)
Hintergrund Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR has a long list of synonyms including FK506 binding protein12 - rapamycin associated protein 1, FK506 binding protein12 - rapamycin associated protein 2, FRAP1, FRAP2, RAFT1, RAPT1 and/or FKBP12-rapamycin associated protein (FRAP). mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gen-ID 2475
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events
Applikationshinweise ELISA: 1/4000 - 1/20000. Western Blot: 1/250 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.40 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Bilder des Herstellers
 image for anti-MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN401320) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody
 image for anti-MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN401320) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody (Image 2)
Allgemeine Veröffentlichungen Chase, Rao, Hofmann, Chang-Hasnain: "1550 nm high contrast grating VCSEL." in: Optics express, Vol. 18, Issue 15, pp. 15461-6, 2010 (PubMed).

Kristof, Marks-Konczalik, Billings, Moss: "Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33637-44, 2003 (PubMed).

Nojima, Tokunaga, Eguchi, Oshiro, Hidayat, Yoshino, Hara, Tanaka, Avruch, Yonezawa: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003 (PubMed).

McMahon, Choi, Lin, Abraham, Lawrence: "The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 21, pp. 7428-38, 2002 (PubMed).

Hudson, Liu, Chiang, Otterness, Loomis, Kaper, Giaccia, Abraham: "Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7004-14, 2002 (PubMed).

Choi, Bertram, Drenan, Carvalho, Zhou, Zheng: "The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase." in: EMBO reports, Vol. 3, Issue 10, pp. 988-94, 2002 (PubMed).