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GLI2 Antikörper (Isoform alpha)

GLI2 Reaktivität: Human, Maus, Ratte, Schimpanse, Rind (Kuh) WB, IHC (p), EIA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN401285
  • Target Alle GLI2 Antikörper anzeigen
    GLI2 (GLI Family Zinc Finger 2 (GLI2))
    Bindungsspezifität
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 40-60, AA 46-60, Isoform alpha
    Reaktivität
    Human, Maus, Ratte, Schimpanse, Rind (Kuh)
    Wirt
    • 40
    • 2
    • 1
    Kaninchen
    Klonalität
    • 41
    • 2
    Polyklonal
    Konjugat
    • 19
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    Dieser GLI2 Antikörper ist unkonjugiert
    Applikation
    • 16
    • 10
    • 9
    • 5
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Spezifität
    This antibody is directed against Gli-2 protein. Expect reactivity with all isoforms of this protein.
    Kreuzreaktivität (Details)
    Species reactivity (expected):Chimpanzee (100 %), Mouse and Rat (93 %), Bovine (86 %).
    Species reactivity (tested):Human.
    Aufreinigung
    Immunoaffinity Chromatography.
    Immunogen
    Synthetic peptide corresponding to amino acids 46-60 of Human Gli-2 (isoform alpha).
    Isotyp
    IgG
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  • Applikationshinweise
    ELISA: 1/2,000-1/12,000. Western blot: 1/500-1/2,000. Detection of Gli-2 may be enhanced if nuclear extracts areused instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced,making it difficult to detect in whole tissue homogenates. Immunohistochemistry on Paraffin Sections: 1/500-1/2,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.1 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    GLI2 (GLI Family Zinc Finger 2 (GLI2))
    Andere Bezeichnung
    GLI2 (GLI2 Produkte)
    Synonyme
    HPE9 antikoerper, THP1 antikoerper, THP2 antikoerper, GLI2/GLI4 antikoerper, GLI3 antikoerper, AW546128 antikoerper, GLI family zinc finger 2 antikoerper, GLI-Kruppel family member GLI2 antikoerper, GLI2 antikoerper, Gli2 antikoerper
    Hintergrund
    Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is a nuclear protein thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes, Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. Multiple splice variants have been reported for this protein.Synonyms: GLI-2, THP, Tax helper protein, Zinc finger protein GLI2
    Gen-ID
    2736
    NCBI Accession
    NP_005261
    UniProt
    P10070
    Pathways
    Hedgehog Signalweg, Dopaminergic Neurogenesis
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