ROBO1 Antikörper (AA 1632-1644)

Produktnummer ABIN401279

Produktdetails anti-ROBO1 Antikörper

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Bindungsspezifität: AA 1632-1644

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ROBO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody detects ROBO-1.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Canine (100 %).
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/30,000-1/160,000. Western blot: 1/500-1/3,000 (Expect a band at approx. 181 kDa in size corresponding to ROBO-1). Immunohistochemistry on Paraffin Sections: 2-10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: SStore the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu ROBO1

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Andere Bezeichnung: ROBO1 (ROBO1 Produkte)

Synonyme: DUTT1 antikoerper, SAX3 antikoerper, AW494633 antikoerper, AW742721 antikoerper, Gm310 antikoerper, ROBO1 antikoerper, dutt1 antikoerper, roundabout antikoerper, sax3 antikoerper, MGC122264 antikoerper, roundabout-1 antikoerper, roundabout guidance receptor 1 antikoerper, roundabout, axon guidance receptor, homolog 1 (Drosophila) antikoerper, roundabout guidance receptor 1 L homeolog antikoerper, ROBO1 antikoerper, Robo1 antikoerper, robo1 antikoerper, robo1.L antikoerper

Hintergrund: ROBO-1 (also called Roundabout homolog 1 precursor and Deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2. The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. ROBO-1 may also be required for lung development. ROBO-1 is a type I membrane protein. ROBO-1 is a widely expressed protein with the exception of the kidney. Defects in ROBO1 may be a cause of breast and lung cancer. ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. Multiple splice variants have been identified for this protein.Synonyms: DUTT1, Deleted in U Twenty Twenty, ROBO-1, Roundabout homolog 1

Gen-ID: 6091

NCBI Accession: NP_002932

UniProt: Q9Y6N7

Pathways: Positive Regulation of Endopeptidase Activity