PARK7/DJ1 Antikörper (AA 177-189)

Produktnummer ABIN401278

Produktdetails anti-PARK7/DJ1 Antikörper

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Bindungsspezifität: AA 177-189

Reaktivität: Human, Affe, Zebrafisch (Danio rerio), Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PARK7/DJ1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: Thisa ntibody is specific for PARK7.

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee, African green monkey, zebrafish and mutant/variant forms of human DJ-1 protein (100 %).. Cross reactivity with frog, mouse, rat, dog, chicken, Japanese rice fish and Atlantic salmon may also cross react as the sequence varies by only one amino acid residue as indicated by BLAST analysis.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding aa 177-189 of Human PARK7 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10,000 - 1: 50,000. Immunohistochemistry on paraffin sections: 2 μg/mL - 5 μg/mL. Western blot: 1: 500 - 1: 2,000 (Expect a band approximately 28 kDa in size correspondingto PARK7 in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu PARK7/DJ1

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Andere Bezeichnung: DJ-1 / PARK7 (PARK7 Produkte)

Synonyme: DJ-1 antikoerper, DJ1 antikoerper, CAP1 antikoerper, Dj1 antikoerper, SP22 antikoerper, dj1 antikoerper, zgc:103725 antikoerper, park7b antikoerper, park7 antikoerper, park7a antikoerper, Parkinsonism associated deglycase antikoerper, Parkinson disease (autosomal recessive, early onset) 7 antikoerper, parkinson protein 7 antikoerper, parkinson protein 7 S homeolog antikoerper, parkinson protein 7 L homeolog antikoerper, PARK7 antikoerper, Park7 antikoerper, park7 antikoerper, park7.S antikoerper, park7.L antikoerper

Hintergrund: The product of the PARK7 gene, known as PARK7, Parkinson disease (autosomal recessive, early onset) 7, DJ-1 mutant, and DJ-1 oncogene product, functions as a positive regulator of androgen receptor-dependent transcription. PARK7 may also function as a redoxsensitive chaperone and as a sensor for oxidative stress and also has been reported to prevent aggregation of SNCA. PARK7 protects neurons against oxidative stress and cell death and plays a role in fertilization. While PARK7 has no proteolytic activity, it does have a weak transforming activity. PARK7 forms a homodimer and binds to DJBP and PIAS2. PARK7 is part of a ternary complex containing PARK7, DJBP and AR and shows both a nuclear and cytoplasmic localization. In some cells, PARK7 is associated with mitochondria, particularly after oxidative stress. This protein is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart tissue, and is expressed at lower levels in placenta and brain. PARK7 is detected in tau inclusions in brains from neurodegenerative disease patients, in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. Defects in PARK7 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, response to treatment with levodopa, the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins) in surviving neurons.Synonyms: Oncogene DJ1, Parkinson disease protein 7

Gen-ID: 11315

NCBI Accession: NP_001116849

UniProt: Q99497

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport