SAE1 Antikörper

Produktnummer ABIN401032

Produktdetails anti-SAE1 Antikörper

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SAE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against SUMO Activating Enzyme E1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 fused with GST

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5,000 - 1/20,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water or equivalent.

Konzentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide, None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Andere Bezeichnung: SAE1 / AOS1 (SAE1 Produkte)

Synonyme: AOS1 antikoerper, HSPC140 antikoerper, SUA1 antikoerper, UBLE1A antikoerper, 2400010M20Rik antikoerper, 2610044L12Rik antikoerper, AL033372 antikoerper, AW743391 antikoerper, D7Ertd177e antikoerper, Sua1 antikoerper, Uble1a antikoerper, Aos1p antikoerper, Sua1p antikoerper, aos antikoerper, sae2a antikoerper, uble1a antikoerper, wu:fa28b04 antikoerper, zgc:86633 antikoerper, SUMO1 activating enzyme subunit 1 antikoerper, SUMO1 activating enzyme subunit 1 L homeolog antikoerper, SAE1 antikoerper, Sae1 antikoerper, sae1.L antikoerper, sae1 antikoerper

Hintergrund: SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) is a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1, also called Aos1) subunit resembles the Nterminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A

Gen-ID: 10055

NCBI Accession: NP_005491

UniProt: Q9UBE0