SARS M (0) (C-Term) Antikörper

Antigen: SARS M (0)

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB), Immunoelectron Microscopy (IEM)

Produktnummer: ABIN400985

Produktbeschreibung

Produktmerkmale: Synonyms: SARS-CoV M, Severe acute respiratory syndrome coronavirus Membrane protein, M protein,Membrane glycoprotein, Matrix protein, Matrix glycoprotein, E1 glycoprotein

Weitere Bezeichnung: SARS M

Swiss-Prot: P59596

Immunogen: BSAcoupled Synthetic peptide corresponding to the C-terminus (amino acid residues204-221) of the SARS Coronavirus Membrane proteinAA Sequence: 1 madngtitve elkqlleqwn lvigflclaw imllqfaysnrnrflyiikl vflwllwpvt61 lacfvlaavy rinwvtggia iamacivglm wlsyfvasfr lfartrsmwsfnpetnilln121 vplrgtivtr plmeselvig aviirghlrm aghslgrcdi kdlpkeitvatsrtlsyykl181 gasqrvgtds gfaaynryri gnyklntdha gsndniallv q

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: The coronavirus membrane protein is a major structural component of the viral envelopeand a determinant of virus assembly.

Spezifität: This antibody is directed against SARS-Coronavirus membrane protein. Cross reactivitywith homologues from other sources has not been determined.

Anwendungen

Applikationshinweise: Immunoprecipitaion: 1: 60. Western blot: 1: 1,000 (a band of approximately 25 kDa in size corresponding to SARS-CoVmembrane protein in the appropriate cell lysate or extract). Immunoelectron microscopy: 1: 200. Immunoflourescence: 1: 1,000 (Vero-E6 cells, grown on glass slides, were infected withSARS-CoV-Fr1 strain for 1 h at 37°C. Infection occurred in PBS/DEAE/2%FCS followed byexchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washingfixed cells, antibody incubation was performed in PBS/5%FCS for 30 min). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 85 mg/ml (by Refractometry)

Reinigung: Serum

Buffer: 0.01% (w/v) Sodium Azide

Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C or below for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar