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DYKDDDDK Tag Antikörper

WB, IP, FACS, EIA Wirt: Maus Monoclonal 29E4-G7-H11 unconjugated
Produktnummer ABIN400788
  • Target Alle DYKDDDDK Tag Produkte
    DYKDDDDK Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 58
    • 52
    • 9
    • 8
    • 6
    Maus
    Klonalität
    • 67
    • 65
    Monoklonal
    Konjugat
    • 47
    • 15
    • 10
    • 7
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser DYKDDDDK Tag Antikörper ist unkonjugiert
    Applikation
    • 80
    • 62
    • 29
    • 24
    • 20
    • 19
    • 12
    • 12
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
    Sequenz
    DYKDDDDK
    Produktmerkmale
    Synonyms: DYKDDDDK Epitope Tag, ECS Epitope Tag, DYKDDDDK-Tag, ECS-tag, D-tag
    Aufreinigung
    Protein A Chromatography followed by extensive dialysis against the buffer.
    Immunogen
    Synthetic peptide corresponding to the DYKDDDDK epitope tag peptide DYKDDDDK(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Remarks: Residues of Glycine and Cysteine were added to the termini to facilitatecoupling.
    Klon
    29E4-G7-H11
    Isotyp
    IgG2a
  • Applikationshinweise
    This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusionproteins. As such, this antibody can be used to identify fusion proteins containing theDYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the amino- orcarboxy- termini of targeted proteins. The epitope tag peptide sequence was first derivedfrom the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Recommended Dilutions:
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Haltbarkeit
    12 months
  • Target
    DYKDDDDK Tag
    Andere Bezeichnung
    DYKDDDDK Epitope Tag (DYKDDDDK Tag Produkte)
    Substanzklasse
    Tag
    Hintergrund
    Sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
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