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AKT1 Antikörper (Ser473)

AKT1 Reaktivität: Human, Maus, Ratte, Schimpanse WB, IF, IHC (p), IP, EIA Wirt: Maus Monoclonal 17F6-B11 unconjugated
Produktnummer ABIN400784
  • Target Alle AKT1 Antikörper anzeigen
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    Bindungsspezifität
    • 98
    • 48
    • 37
    • 34
    • 32
    • 22
    • 20
    • 19
    • 19
    • 17
    • 16
    • 11
    • 11
    • 9
    • 9
    • 9
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Ser473
    Reaktivität
    • 562
    • 367
    • 288
    • 55
    • 54
    • 46
    • 34
    • 20
    • 12
    • 12
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Schimpanse
    Wirt
    • 481
    • 101
    • 7
    • 2
    • 1
    Maus
    Klonalität
    • 433
    • 158
    Monoklonal
    Konjugat
    • 319
    • 39
    • 28
    • 25
    • 25
    • 19
    • 16
    • 16
    • 14
    • 13
    • 12
    • 12
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Dieser AKT1 Antikörper ist unkonjugiert
    Applikation
    • 448
    • 258
    • 193
    • 87
    • 82
    • 75
    • 56
    • 52
    • 31
    • 29
    • 28
    • 15
    • 12
    • 9
    • 9
    • 9
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    Spezifität
    This phospho-specific monoclonal antibody reacts with Human and Mouse AKT pSer473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Cross-reactivity with AKT2 and AKT3 has not been determined.
    Kreuzreaktivität (Details)
    Species reactivity (expected):Mouse, Chimpanzee, Rat.
    Species reactivity (tested):Human.
    Produktmerkmale
    Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase
    Aufreinigung
    Protein A Chromatography
    Immunogen
    Synthetic peptide corresponding to residues surrounding Ser473 of Human AKT1 protein
    Klon
    17F6-B11
    Isotyp
    IgG1
    Top Product
    Discover our top product AKT1 Primärantikörper
  • Applikationshinweise
    ELISA: 1/20,000. Immunoprecipitation. Immunoflourescence: 1/500-1/3,000. Western blot: 1/500-1/3,000 (Expect a band approximately 56 kDa in size corresponding tophosphorylated AKT protein by in the appropriate cell lysate or extract). Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 20 μg/mL (Nopre-treatment of sample is required).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.02 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
    Shelf life: one year from despatch.
    Haltbarkeit
    12 months
  • Target
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    Andere Bezeichnung
    AKT1 / PKB (AKT1 Produkte)
    Synonyme
    AKT antikoerper, CWS6 antikoerper, PKB antikoerper, PKB-ALPHA antikoerper, PRKBA antikoerper, RAC antikoerper, RAC-ALPHA antikoerper, Akt antikoerper, Rac antikoerper, PKB/Akt antikoerper, PKBalpha antikoerper, AKT/PKB antikoerper, AKT1 antikoerper, Akt/PKB antikoerper, Akt1 antikoerper, CG4006 antikoerper, D-Akt antikoerper, DAKT1 antikoerper, DAKT1/PKB antikoerper, DAkt antikoerper, DAkt1 antikoerper, DPKB antikoerper, DRAC-PK antikoerper, DRAC-PK66 antikoerper, DRAC-PK85 antikoerper, Dakt antikoerper, Dakt1 antikoerper, Dmel\\CG4006 antikoerper, Dpkb antikoerper, PKB/AKT antikoerper, PKB/dAKT antikoerper, RacPK antikoerper, akt antikoerper, akt1 antikoerper, dAKT antikoerper, dAKT/dPKB antikoerper, dAKT1 antikoerper, dAkt antikoerper, dAkt/PKB antikoerper, dAkt1 antikoerper, dPKB antikoerper, dakt antikoerper, dakt1 antikoerper, l(3)04226 antikoerper, l(3)89Bq antikoerper, p-Akt antikoerper, pAkt antikoerper, ACT-5 antikoerper, akt-1 antikoerper, pkb antikoerper, v-akt antikoerper, v-akt1 antikoerper, xAct antikoerper, ATAKT1 antikoerper, F18A8.2 antikoerper, F18A8_2 antikoerper, K+ transporter 1 antikoerper, POTASSIUM TRANSPORTER antikoerper, AKT serine/threonine kinase 1 antikoerper, thymoma viral proto-oncogene 1 antikoerper, CG4006 gene product from transcript CG4006-RE antikoerper, actin beta antikoerper, v-akt murine thymoma viral oncogene homolog 1 S homeolog antikoerper, v-akt murine thymoma viral oncogene homolog 1 antikoerper, K+ transporter 1 antikoerper, Serine/threonine-protein kinase akt-1 antikoerper, AKT1 antikoerper, Akt1 antikoerper, ACTB antikoerper, akt1.S antikoerper, akt1 antikoerper, KT1 antikoerper, akt-1 antikoerper
    Hintergrund
    AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
    Gen-ID
    207
    UniProt
    P31749
    Pathways
    PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt
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