Nestin (NES) Antikörper
| Antigen |
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| Synonyme |
FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (86), Maus (37), Ratte (Rattus) (33), Rind (Kuh) (7), Kaninchen (7), Hund (6), Pferd (4), Schwein (4), Hamster (2), Huhn (1), Schimpanse (1), Affe (1), Schaf (1)
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Applikation
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Western Blot (WB) (70), Immunfluoreszenz (IF) (61), Durchflusszytometrie (FACS) (50), Immunhistochemie (Paraffinschnitte) (IHC (p)) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (11), Immunhistochemie (IHC) (11), Enzyme Immunoassay (EIA) (10), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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| Produktnummer |
ABIN398889 |
| Menge |
40ug (0.5 mg/ml, lyophilized with PBS, pH...) (Varianten) |
| Preis |
76,92 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Weitere Bezeichnung
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Nestin
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Format
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Affinity-purified
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Isotyp
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IgG (Passende Sekundärantikörper)
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Beschreibung
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Nestin is a class IV intermediate filament protein which is abundantly expressed in neuroepithelial stem cells early in embryogenesis. It contains a short head domain and a long tail domain. Upon differentiation, nestin is down regulated and is replaced by other tissue specific intermediate filaments. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS.Antibodies-Online Rabbit Anti-Nestin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from within residues 1280-1330 of human Nestin (Swiss Prot: P48681).
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Spezifität
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Antibodies-Online Rabbit Anti-Nestin Polyclonal Antibody detects endogenous levels of human Nestin. It is predicted to react with mouse and rat according to sequence homology.
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Applikationshinweise
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-1 ug/mlOther Applications: user-optimized
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Konzentration
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0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
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Reinigung
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Immunoaffinity chromatography
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Lagerung
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Rabbit Anti-Nestin Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.
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Forschungsgebiet
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Angiogenese, Abstammungsmarker
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Nestin (NES)", Typ "Antikörper" finden
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Wirte
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Kaninchen (80), Maus (30), Huhn (3), Ziege (1)
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Reaktivitäten
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Human (86), Maus (37), Ratte (Rattus) (33), Rind (Kuh) (7), Kaninchen (7), Hund (6), Pferd (4), Schwein (4), Hamster (2), Huhn (1), Schimpanse (1), Affe (1), Schaf (1)
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Applikationen
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Western Blot (WB) (70), Immunfluoreszenz (IF) (61), Durchflusszytometrie (FACS) (50), Immunhistochemie (Paraffinschnitte) (IHC (p)) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (11), Immunhistochemie (IHC) (11), Enzyme Immunoassay (EIA) (10), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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Konjugate
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Biotin (4), HRP (4), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitope
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C-Term (4), AA 1484-1500 (1), AA 544-776 (1)
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