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Antikörper
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anti-Glial Fibrillary Acidic Protein (GFAP) Antikörper
Glial Fibrillary Acidic Protein (GFAP) Antikörper
| Antigen |
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| Synonyme |
GFAP, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345, gfapl, MGC110485, wu:fb34h11, wu:fk42c12, zgc:110485, etID36982.3, xx:af506734 |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (203), Ratte (Rattus) (92), Schwein (72), Maus (69), Rind (Kuh) (53), Schaf (26), Huhn (25), Kaninchen (21), Hund (19), Meerschweinchen (17), Katze (13), Säugetiere (13), Affe (7), Hamster (6), Vogel (3), Ziege (3), Caprinae (2), Pferd (1), Primate (1)
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Applikation
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 Alternativen ELISA, Western Blot (WB), Immunhistochemie (IHC)
Western Blot (WB) (192), Immunhistochemie (Paraffinschnitte) (IHC (p)) (133), Immunfluoreszenz (IF) (118), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (93), Immunhistochemie (IHC) (47), Immunpräzipitation (IP) (40), Durchflusszytometrie (FACS) (24), Enzyme Immunoassay (EIA) (23), Immunzytochemie (ICC) (23), ELISA (18), ELISA (Detektionsantikörper) (3), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Fangantikörper) (1), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN398827 |
| Menge |
40ug (0.5 mg/ml, lyophilized with PBS, pH...) (Varianten) |
| Preis |
76,92 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Weitere Bezeichnung
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GFAP
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Format
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Affinity-purified
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Isotyp
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IgG (Passende Sekundärantikörper)
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Beschreibung
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GFAP (Glial fibrillary acidic protein) is a member of intermediate filament (IF) protein family which consists mainly of cytokeratin, GFAP, desmin, and vimentin. Like other IF proteins, GFAP is composed of an amino terminal head domain, a central rod domain, and a carboxyl terminal tail domain. The amino terminal region of GFAP shares considerable sequence divergence with other IF proteins, and probably participates in mediation of the tissue specific functions. GFAP intermediate filament serves as a useful marker of astroglia in the brain since it is specifically expressed in astrocytes and certain other astroglia. This protein may be required for communications between Bergmann glia and Purkinje cells during long-term depression induction and maintenance.Antibodies-Online Rabbit Anti-GFAP Polyclonal Antibody is developed in rabbit using a synthetic KLH-coupled peptide from within 250-300 of human GFAP (Swiss Prot: P14136).
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Spezifität
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Antibodies-Online Rabbit Anti-GFAP Polyclonal Antibody detects endogenous levels of human and rat GFAP. It is predicted to react with sumatran orangutan GFAP protein according to sequence homology.
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Applikationshinweise
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry: 5-10 ug/mlOther Applications: user-optimized
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Konzentration
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0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
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Lagerung
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Rabbit Anti-GFAP Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.
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Forschungsgebiet
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Stammzellen, Zytoskelett, Glia Marker, Zell-/Gewebemarker, Neurologie
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Glial Fibrillary Acidic Protein (GFAP)", Typ "Antikörper" finden
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Wirte
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Maus (185), Kaninchen (96), Huhn (15), Ziege (6), Meerschweinchen (2)
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Reaktivitäten
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Human (203), Ratte (Rattus) (92), Schwein (72), Maus (69), Rind (Kuh) (53), Schaf (26), Huhn (25), Kaninchen (21), Hund (19), Meerschweinchen (17), Katze (13), Säugetiere (13), Affe (7), Hamster (6), Vogel (3), Ziege (3), Caprinae (2), Pferd (1), Primate (1)
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Applikationen
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Western Blot (WB) (192), Immunhistochemie (Paraffinschnitte) (IHC (p)) (133), Immunfluoreszenz (IF) (118), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (93), Immunhistochemie (IHC) (47), Immunpräzipitation (IP) (40), Durchflusszytometrie (FACS) (24), Enzyme Immunoassay (EIA) (23), Immunzytochemie (ICC) (23), ELISA (18), ELISA (Detektionsantikörper) (3), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Fangantikörper) (1), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Biotin (7), Cy3 (4), HRP (4), Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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pSer8 (2), C-Term (1), internal (1)
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