Aha1 Antikörper

Antigen: Aha1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus), Maus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN398792

Produktbeschreibung

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: AHA1 is a cochaperone that stimulates HSP90 ATPase activity. It may also affect a step in the endoplasmic reticulum with regard to Golgi trafficking. AHA1 interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatitis virus glycoprotein. The protein is expressed in numerous tissues, including brain, heart, skeletal muscle, and kidney tissues and, at lower levels, liver and placental tissues.Antibodies-Online Rabbit Anti-AHA1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 50-100 of human AHA1 protein (Swiss Prot: O95433).

Spezifität: Antibodies-Online Rabbit Anti-AHA1 Polyclonal Antibody detects endogenous level of human,rat, and mouse AHA1 protein.

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-1 ug/mlOther applications: user optimized

Konzentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Reinigung: Immunoaffinity chromatography

Lagerung: Rabbit Anti-AHA1 Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Forschungsgebiet: Signaltransduktion, Hitzeschockproteine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Lane 1: Mouse brain lysateLane 2: Rat brain lysateWestern blot analysis of tissue lysates using 1 ug/ml Rabbit Anti-AHA1 Polyclonal Antibody (Antibodies-Online, ABIN398793)Predicted band size 38 KDObserved band size 38 KDAdditional band size 25 KDThe signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Lane 1 and 3: A549 lysateLane 2 and 4: K562 lysate Western blot analysis of cell lysates using 1 ug/ml Rabbit Anti-AHA1 Polyclonal Antibody (Antibodies-Online, ABIN398793)Predicted band size 38 KDObserved band size 41 KDAdditional band size 25 KDThe signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.