Tubulin, Beta, 3 (TUBB3) Antikörper

Antigen: Tubulin, Beta, 3 (TUBB3)

Synonyme: TUBB3, TUBB4, MGC134115, MC1R, beta-4, tbb3, M(beta)3, M(beta)6, 3200002H15Rik, tubb3, MGC76202, TUBB1, tubB3, tubulin

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunhistochemie (IHC), Durchflusszytometrie (FACS)

Produktnummer: ABIN398739

Produktbeschreibung

Weitere Bezeichnung: beta-Tubulin III

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Beta-Tubulin III belongs to the tubulin family. Tubulin is the major constituent of microtubules. It forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. beta-Tubulin III contains a highly acidic C-terminal region which can bind cations such as calcium. It is abundant in the central and peripheral nervous systems.Antibodies-Online Rabbit Anti-beta-Tubulin III Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 150-200 of human beta-Tubulin III protein (Swiss Prot: Q13509).

Spezifität: Antibodies-Online Rabbit Anti-beta-Tubulin III Polyclonal Antibody detects endogenous levels of human, rat and mouse beta-tubulin III protein. Sequence homology predicts that it will also react with bovine, chicken, cynomolgus monkey. African clawed frog, and disc abalone beta-tubulin III proteins. Reactivity with other β-Tubulin had not been tested yet.

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 ug/ml. Western blot: 0.5-1 ug/ml. Immunohistochemistry: 5-10 ug/ml. Flow cytometry: 1-3 ug for 1 x 10E6 cells. Other applications: user-optimized.

Konzentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Reinigung: Immunoaffinity chromatography

Lagerung: Rabbit Anti-beta-Tubulin III Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Lane 1: MarkerLane 2: Hela cell lysateLane 3: HEK293 cell lysateLane 4: Mouse brain tissue lysateLane 5: Rat brain tissue lysateWestern blot analysis of lysates using 1 ug/ml Rabbit Anti-beta-Tubulin III Polyclonal Antibody (Antibodies-Online, ABIN398739)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded) using Rabbit Anti-beta-Tubulin III Polyclonal Antibody (Antibodies-Online, ABIN398739)

Immunohistochemistry negative control analysis of human brain tissue slide (Paraffin embedded) using Purified Rabbit IgG (Whole Molecule) Control (Antibodies-Online, ABIN398653)