Catalase (CAT) Antikörper

Antigen: Catalase (CAT)

Synonyme: MGC138422, MGC138424, Cas1, Cs-1, Cas-1, 2210418N07, CS1, RATCATL, RATCAT01, fb68a12, wu:fb68a12, CAT, CATA, CT21282, DMCATHPO, DROCATHPO, U00145, bs36h11.y1, DmelCG6871, CG6871, MGC128112, CATALASE, CATALASE 2, T12J5.2, Cat, GB11648, catalase, MGC147040, DKFZp469E0232

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP), Durchflusszytometrie (FACS)

Produktnummer: ABIN398710

Produktbeschreibung

Weitere Bezeichnung: Catalase

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Catalase, also known as CAT, is the classical marker for peroxisomes, in which it is the most abundant protein. It is found in almost all aerobically respiring organisms and serves to protect cells from hydrogen peroxide toxicity. Defects in CAT cause acatalasia, which is characterized by the absence of catalase activity in red cells and is often associated with ulcerating oral lesions.Antibodies-Online Catalase Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide derived from the residues near the carboxyl terminus of the human catalase protein (Swiss Prot: P04040).

Spezifität: Antibodies-Online Catalase Antibody, pAb, Rabbit detects endogenous levels of human and mouse catalase protein.

Synonyme: MGC138422, MGC138424, Cas1, Cs-1, Cas-1, 2210418N07, CS1, RATCATL, RATCAT01, fb68a12, wu:fb68a12, CAT, CATA, CT21282, DMCATHPO, DROCATHPO, U00145, bs36h11.y1, DmelCG6871, CG6871, MGC128112, CATALASE, CATALASE 2, T12J5.2, Cat, GB11648, catalase, MGC147040, DKFZp469E0232

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/ml Western blot: 0.5-2 ug/mlImmunoprecipitation (IP): 2-10 ug/mg of lysateFlow cytometry: 1-3 ug for 1 x 106 cellsOther applications: user optimized

Konzentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Reinigung: Immunoaffinity chromatography

Lagerung: Catalase Antibody, pAb, Rabbit should be lyophilized for storage until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Forschungsgebiet: Krebs, Organelle, Hitzeschockproteine, Zell-/Gewebemarker

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of mouse liver tissue lysate using 1 ug/ml Rabbit Anti-Catalase Polyclonal Antibody (Antibodies-Online, ABIN398711)Lane 1. Rabbit Anti-Catalase Polyclonal AntibodyLane 2. Rabbit Anti-Catalase Polyclonal Antibody pre-incubated with immunizing peptideThe signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot analysis of Hela cell lysate using 2 ug/ml Rabbit Anti-Catalase Polyclonal Antibody (Antibodies-Online, ABIN398711)Lane 1. Rabbit Anti-Catalase Polyclonal AntibodyLane 2. Rabbit Anti-Catalase Polyclonal Antibody pre-incubated with immunizing peptideThe signal was developed with IRDye-800 Conjugated Goat Anti-Rabbit IgG.

Western blot analysis of immunoprecipitates from mouse liver lysate using Rabbit Anti-Catalase Polyclonal Antibody (Antibodies-Online, ABIN398711).Lane 1: Input control material for mouse liver lysateLane 2: Negative control-IP with isotype control antibody (Antibodies-Online, ABIN398653)Lane 3: Immunprecipitation with Rabbit Anti-Catalase Polyclonal Antibody (Antibodies-Online, ABIN398711).