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Antikörper
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anti-G Protein-Coupled Estrogen Receptor 1 (GPER) Antikörper
G Protein-Coupled Estrogen Receptor 1 (GPER) Antikörper
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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 Alternativen ELISA, Western Blot (WB), Immunhistochemie (IHC)
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| Produktnummer |
ABIN398686 |
| Menge |
200ug (0.5 mg/ml, lyophilized with PBS, pH...) (Varianten) |
| Preis |
272,73 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Weitere Bezeichnung
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GPR30
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Format
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Affinity-purified
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Isotyp
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IgG (Passende Sekundärantikörper)
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Beschreibung
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GPR30 (G Protein-coupled Receptor 30), also known as G Protein-coupled estrogen Receptor 1 (GPER), is a member of G protein-coupled receptor family. It contains seven hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops. GPR30 transduces a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GPR30 is an orphan receptor and localizes to the endoplasmic reticulum, where it specifically binds estrogen and estrogen derivatives. Activating GPR30 via estrogen results in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. Antibodies-Online Rabbit Anti-GPR30 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from amino terminal of human GPR30 protein (Swiss Prot: Q99527).
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Spezifität
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Antibodies-Online Rabbit Anti-GPR30 Polyclonal Antibody detects endogenous levels of human GPR30 protein.
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Applikationshinweise
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-1 ug/mlIHC: 5-10 ug/mlOther applications: user optimized.
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Konzentration
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0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
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Reinigung
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Immunoaffinity chromatography
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Lagerung
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Rabbit Anti-GPR30 Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "G Protein-Coupled Estrogen Receptor 1 (GPER)", Typ "Antikörper" finden
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Wirte
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Kaninchen (36)
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Reaktivitäten
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Human (36), Maus (23), Ratte (Rattus) (23), Rind (Kuh) (17), Huhn (16), Pferd (15), Kaninchen (15), Hund (2), Schwein (1), Kugelfisch (1)
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Applikationen
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Immunfluoreszenz (IF) (27), Durchflusszytometrie (FACS) (20), Western Blot (WB) (13), Immunhistochemie (Paraffinschnitte) (IHC (p)) (8), ELISA (4), Immunhistochemie (IHC) (3), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunpräzipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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N-Term (1)
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