ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2) Antikörper

Antigen: ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2)

Synonyme: HO57, VATB, VPP3, Vma2, ATP6B2, ATP6B1B2

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN398624

Produktbeschreibung

Weitere Bezeichnung: V-ATPase Subunit B 2

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: The V-ATPase subunit B 2 is related to a gene that encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.Antibodies-Online Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody is developed in rabbit using a synthetic peptide conjugated to KLH. It is purified from rabbit antiserum by immunoaffinity chromatography and supplied as 40 ug aliquot at concentration of 0.5 mg/ml.

Spezifität: Antibodies-Online Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody reacts with human Anti-V-ATPase subunit B. Sequence homology predicts that it will also react with mouse, rat, chicken, bovine, and fruit fly V-ATPase subunit B 2.

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.03-0.125 ug/ml Western blot: 1.0 ug/ml Other applications: user-optimized

Konzentration: 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide

Lagerung: Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody remains stable for 2-3 weeks if stored at 4°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis:Lane 1: EasyWestern Protein Standard (Antibodies-Online, MM0908)Lane 2. Hela cell lysatePrimary antibody: 1 µg/ml Rabbit Anti-ATPase Subunit B 2 Polyclonal Antibody (Antibodies-Online, ABIN398624)Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398323, 1:5,000)