Presenilin 1 (PSEN1) Antikörper

Antigen: Presenilin 1 (PSEN1)

Synonyme: AD3, FAD, PS1, S182, Ad3h, PS-1, pre1, psen, zfPS1, zf-PS1, PSEN1, ad3, fad, ps1, s182, X-PS-beta, X-PS-alpha, DKFZp469K2026

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN398552

Produktbeschreibung

Weitere Bezeichnung: Presenilin 1

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Presenilin 1 (PS1) and presenilin 2 (PS2) are catalytic subunits of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). The other members of the gamma-secretase complex are required to have protease activity. PS1 and PS2 may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It regulates epithelial-cadherin function. Three causative genes have been identified that when mutated lead to presenile Alzheimer's disease: APP (amyloid precursor protein gene), PSEN1, and PSEN2. The presenilin 1 gene has recently been discovered on chromosome 14 and is responsible for an early onset form of Alzheimer's disease that accounts for 70-80% of the inherited forms of Alzheimer's. It is a membrane protein that has a region where at least seven mutations have been identified in family members with Alzheimer's disease. Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (28 kD) and carboxy-terminal (~17 kD) fragments.Antibodies-Online Rabbit Anti-Presenilin 1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide, TVRSQNDNRERQEHNDRRSLGHPEPL, corresponding to amino acids 25-50 of the N-terminus of human presenilin 1.Rabbit Anti-Presenilin 1 Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 0.5 mg/ml.

Spezifität: Antibodies-Online Rabbit Anti-Presenilin 1 Polyclonal Antibody can detect endogenous levels of the full-length protein (approx. 43-52 kD) and the N-terminal fragment of presenilin 1 (~ 28 kD).

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.5-2.0 ug/ml

Konzentration: 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide

Reinigung: Immunoaffinity chromatography

Lagerung: Rabbit Anti-Presenilin 1 Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Forschungsgebiet: Krebs, Alzheimer-Krankheit (Morbus Alzheimer), Alzheimer-Krankheit (Morbus Alzheimer)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Lane 1: Mouse brain tissue lysateLane 2: Hela cell lysatePrimary antibody: 2 ug/ml Rabbit Anti-Presenilin 1 Polyclonal Antibody (Antibodies-Online, ABIN398552)Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398323, 1:5,000)The signal was developed with LumiSensorTM HRP Substrate Kit (Antibodies-Online, L00221V500)