S-Tag Antikörper

Antigen: S-Tag

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN398447

Produktbeschreibung

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Antibodies-Online Rabbit Anti-S-tag Polyclonal Antibody is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. It is purified by immunoaffinity chromatography.

Spezifität: Antibodies-Online Rabbit Anti-S-tag Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal S tagged fusion proteins.

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.1-1.0 ug/mlWestern blot using ONE-HOUR WesternTM Kit:For quick results, Antibodies-Online ONE-HOUR WesternTM Kit L00204C is recommended. 10 ug of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized

Konzentration: 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide

Reinigung: Immunoaffinity chromatography

Lagerung: Rabbit Anti-S-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Forschungsgebiet: Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Lane 1-2: S-tag fusion protein in CHO cell lysate (~52kD)Lane 3: Negative CHO cell lysatePrimary antibody: 1 µg/ml Rabbit Anti-S-tag Polyclonal Antibody (Antibodies-Online, ABIN398447)Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398323, 1:10,000)The signal was developed with LumiSensorTM HRP Substrate Kit (Antibodies-Online, L00221V500)

Loading: S-tag fusion protein expressed in E. coli cell lysatePrimary antibody: 1 µg/ml Rabbit Anti-S-tag Polyclonal Antibody (Antibodies-Online, ABIN398447)The result was developed with One-Step WesternTM Complete Kit (Rabbit) (Antibodies-Online, L00204C)

Western blot analysis of S-tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-S-tag Polyclonal Antibody (Antibodies-Online, ABIN398447, 1 ug/ml)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.