Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antikörper

Antigen: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Synonyme: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Maus, Ratte (Rattus), Ziege, Kaninchen, Huhn, Hamster, Rind (Kuh), Schwein, Fisch, Escherichia Coli (E. Coli)

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN398423

Produktbeschreibung

Weitere Bezeichnung: GAPDH

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often used as a control in experiments quantifying specific changes in expression levels of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes, such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimer-disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.Antibodies-Online Goat Anti-GAPDH Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography. The antibody is supplied as a 40 µg aliquot at a concentration of 1 mg/ml.

Spezifität: Antibodies-Online Goat Anti-GAPDH Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species.

Synonyme: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.1-1.0 ug/mlOther applications: user-optimized

Konzentration: 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide

Reinigung: Immunoaffinity chromatography

Lagerung: Goat Anti-GAPDH Polyclonal Antibody remains stable for 2-3 weeks if stored at 2-8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles.

Forschungsgebiet: Krebs, Metabolismus, Alzheimer-Krankheit (Morbus Alzheimer), Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of GAPDH using Goat Anti-GAPDH Polyclonal Antibody (Antibodies-Online, ABIN398423).Lane 1-5: 5.0 ug, 2.5 ug, 1.25 ug, 0.62 ug and 0.31 ug of Hela cell lysate (Antibodies-Online, M0045).The blot was developed with One-Step WesternTM Blot Kit (Antibodies-Online, L00228).

Fluorescent Western blots for the detection of GAPDH using the One-Step WesternTM Fluorescent Kit (Antibodies-Online, L00397). Lane 1-8: 5.0, 2.5, 1.25, 0.62, 0.31, 0.16, 0.08 and 0.04 ug of HeLa cell lysate.

Western blot analysis of cell lysates using 0.1 ug/ml Goat Anti-GAPDH Polyclonal Antibody (Antibodies-Online, ABIN398423)Lane 1: Hela lysateLane 2: HEK293 lysateLane 3: NIH/3T3 lysateLane 4: LoVo lysateLane 5: HT29 lysateSecondary antibody: Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398411, 1:10,000)The signal was developed with LumiSensorTM HRP Substrate Kit (Antibodies-Online, L00221V500)