Huntingtin (HTT) Antikörper

Antigen: Huntingtin (HTT)

Synonyme: HD, IT15, Hd, Hdh, AI256365, C430023I11Rik, hd, ZHD, HTT, HsapHD, dhtt, DmelCG9995, CG9995, Htt, HDH, SLC6A4, it15, MGC79590, htt, LOC100216476

Klonalität: Monoklonal (A00089-01)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN398314

Produktbeschreibung

Weitere Bezeichnung: Huntingtin

Format: Affinity-purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: A00089-01

Beschreibung: Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10 to 20 years. The HD gene product is widely expressed in human tissues, with the highest level of expression in the brain. The huntingtin gene product is expressed at similar levels in patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using a yeast two-hybrid system, researchers have identified a protein, HAP1 (huntingtin associated protein 1), that associates with huntingtin protein. The in vitro data suggest that the association between HAP1 and huntingtin is enhanced by increasing the length of the glutamine repeat.

Spezifität: Recognize hungtingtin from human and mouse

Anwendungen

Applikationshinweise: Western blot and IHC in lipid metabolic research. Huntingtin is a 350,000 Da protein that is mutated in Huntington's disease (HD). The normal function of huntingtin still remains uncertain but it has been suggested that it performs a necessary housekeeping function. The abnormal form of huntingtin aggregates in vitro and forms neuronal intranuclear and cytoplasmic inclusions in HD patients. This antibody detects a 350,000 Da band on Western blots but also detects smaller degradation products of huntingtin in some tissue lysates.

Konzentration: 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid

Reinigung: Immunoaffinity purification

Lagerung: The antibody will remain stable for one year from the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. The aliquot will be remain stable for three months if stored at 4°C.

Forschungsgebiet: Neurologie, Virologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Huntingtin (HTT) antibody