Green Fluorescent Protein (GFP) Antikörper

Antigen: Green Fluorescent Protein (GFP)

Klonalität: Monoklonal (3H9)

Wirt: Ratte

Applikation: Western Blot (WB), ELISA

Produktnummer: ABIN398304

Produktbeschreibung

Produktmerkmale: Rat monoclonal [3H9] to Green Fluorescent Proteins. For biochemical and cell biological analysis of GFP-tagged fusion proteins.

Format: Supernatant

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: 3H9

Beschreibung: Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria. Here we offer a rat monoclonal antibody (MAb) against green FPs, designated as 3H9. The 3H9 MAb is characterized by high affinity and specificity against GFP. It is suitable for immunoblotting and ELISA. With this versatile antibody GFP and fusions thereof become attractive tools for biochemical applications like identification of interacting proteins or Western Blotting.

Anwendungen

Applikationshinweise: Western Blot: Used at a dilution of 1/1000. Detects a band of approximately 31 kDa (the predicted molecular weight of GFP).

Konzentration: 1mg/ml

Reinheit: Liquid. hybridoma supernatant. The hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kD exclusion membrane to an antibody concentration of 1 mg/ml.

Buffer: Preservative: 0.01% Sodium Azide

Lagerung: Shipped at RT. Upon delivery aliquot and store at -20°C. Store at 4°C short term (4-8 weeks). Avoid freeze / thaw cycles.

Forschungsgebiet: Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blot analysis using anti-GFP antibody 3H9. Primary antibody: 0.2 ug of GFP antibody 3H9 incubation for 1 h at RT. Secondary antibody: anti-rat-HRP (Jackson ImmunoResearch Europe). Detection with ECL Western Blot Detection Kit

Publikationen

Publikationen: Sieber, Lange, Kollmorgen et al.: "Sharpin Contributes to TNFα Dependent NFκB Activation and Anti-Apoptotic Signalling in Hepatocytes." in: PLoS ONE, Vol. 7, Issue 1, pp. e29993, 2012 (PubMed).