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Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) (AA 1-104) Antikörper

Details zu Produkt Nr. ABIN395003, Anbieter: Anmelden zum Anzeigen
Antigen
  • cstf3
  • MGC76035
  • CSTF3
  • wu:fa99c02
  • wu:fb18b09
  • zgc:56028
  • 4732468G05Rik
  • C79532
  • CstF-77
  • cstf3a
  • CSTF-77
Epitop
AA 1-104
17
15
4
3
3
2
2
2
2
1
1
1
Reaktivität
Human
64
23
23
6
3
2
2
2
1
1
1
Wirt
Maus
50
10
4
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Western Blotting (WB)
49
30
11
11
6
6
3
1
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Immunogen CSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Klon 1D4
Isotyp IgG1 kappa
Spezifität CSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Reinigung This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Andere Bezeichnung CSTF3 (CSTF3 Antibody Abstract)
Hintergrund Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molekulargewicht 82922 DA
Gen-ID 12406
Forschungsgebiet Cell Structure
Pathways
Applikationshinweise Western blot = 1:500-1000
Kommentare

Background: The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer PBS with 0.09% (w/v) sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Haltbarkeit 6 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) (AA 1-104) Antikörper (ABIN395003) Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) (AA 1-104) Antikörper (ABIN395003) anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) (AA 1-104) antibody (Image 2)
Allgemeine Veröffentlichungen Hockert, Yeh, MacDonald: "The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation." in: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 695-704, 2009 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: , Vol. 3, pp. 89, 2007 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Taylor, Noguchi, Totoki et al.: "Human chromosome 11 DNA sequence and analysis including novel gene identification. ..." in: Nature, Vol. 440, Issue 7083, pp. 497-500, 2006 (PubMed).

Kim, Bhinge, Morgan et al.: "Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment." in: Nature methods, Vol. 2, Issue 1, pp. 47-53, 2005 (PubMed).