AKT3 (N-Term) Antikörper

Antigen: AKT3

Epitope: N-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391025

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: AKT3 (N-term)

Gen-ID: 10000

Swiss-Prot: Q9Y243

Immunogen: This AKT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~127 amino acids from the N-terminal region of human AKT3 Antibody.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, Protein kinase Akt-3, Protein kinase B, gamma, PKB gamma, STK-2, AKT3,

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AKT3 Antibody.

Molekulargewicht: 55775 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50-100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Krebs, Zellzyklus, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of anti-AKT3 Antibody (N-term) Pab (ABIN391025) in Hela cell line lysates (35ug/lane). AKT3(arrow) was detected using the purified Pab (1:30 dilution).

Western blot analysis of anti-AKT3 Antibody (N-term) Pab (ABIN391025) in 293 cell line lysates transiently transfected with the AKT3 gene (2ug/lane). AKT3(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with AKT3 Antibody (N-term)(7030a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.