WEE1 Homolog (S. Pombe) (WEE1) (pS53) Antikörper

Antigen: WEE1 Homolog (S. Pombe) (WEE1)

Synonyme: WEE1A, WEE1hu, FLJ16446, DKFZp686I18166, wee1a, xwee1, MGC81696, xe-wee1a, wee1hu, wee1B, WEE1, MGC82009, ARABIDOPSIS WEE1 KINASE HOMOLOG, ATWEE1, F22D16.3, F22D16_3, WEE1-KINASE, GB19689

Epitope: pS53

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), Dot Blot (Dot), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN389682

Produktbeschreibung

Produktmerkmale: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: Phospho-Wee1-S53

Gen-ID: 7465

Swiss-Prot: P30291

Immunogen: This Phospho-Wee1-S53 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S53 of human WEE1

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Wee1-like protein kinase, Wee1A kinase, WEE1hu, WEE1,

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S53 of human WEE1

Molekulargewicht: 71597 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Dot Blot ~~ 1:500

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Zellzyklus, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Dot blot analysis of anti-Phospho-Wee1-S53 Antibody (ABIN389682) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.

The anti-Phospho-Wee1-S53 Pab (ABIN389682) is used in Western blot to detect Phospho-Wee1-S53 in mouse liver tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma