LC3B Antikörper (cleaved)

Produktnummer ABIN388484

Produktdetails anti-LC3B Antikörper

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Bindungsspezifität: AA 89-122, cleaved

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LC3B Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC)

Homologie: B

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.

Klon: RB15839-RB28608

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:100. IF: 1:10~50. ICC: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-LC3B Antikörper (ABIN388484)

Ock, Park, Han, Jeong, Kim, Lee, Hahm: "Genetic ablation or pharmacologic inhibition of autophagy mitigated NSAID-associated gastric damages." in: Journal of molecular medicine (Berlin, Germany), Vol. 95, Issue 4, pp. 405-416, (2018) (PubMed).

Xu, Huai, Meng, Dong, Liu, Qi, Hu, Fan, Jin, Lv: "L-3-n-Butylphthalide Activates Akt/mTOR Signaling, Inhibits Neuronal Apoptosis and Autophagy and Improves Cognitive Impairment in Mice with Repeated Cerebral Ischemia-Reperfusion Injury." in: Neurochemical research, Vol. 42, Issue 10, pp. 2968-2981, (2018) (PubMed).

Filipczak, Thomas, Chen, Salzman, McDonald, Lin, Belinsky: "TSC2 Deficiency Unmasks a Novel Necrosis Pathway That Is Suppressed by the RIP1/RIP3/MLKL Signaling Cascade." in: Cancer research, Vol. 76, Issue 24, pp. 7130-7139, (2017) (PubMed).

Lee, Park, Hahm: "Mitigated NSAID-induced apoptotic and autophagic cell death with Smad7 overexpression." in: Journal of clinical biochemistry and nutrition, Vol. 60, Issue 1, pp. 55-62, (2017) (PubMed).

Jutten, Keulers, Schaaf, Savelkouls, Theys, Span, Vooijs, Bussink, Rouschop: "EGFR overexpressing cells and tumors are dependent on autophagy for growth and survival." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 108, Issue 3, pp. 479-83, (2013) (PubMed).

Radtke, English, Rondeau, Leib, Lippé, Desjardins: "Inhibition of the host translation shutoff response by herpes simplex virus 1 triggers nuclear envelope-derived autophagy." in: Journal of virology, Vol. 87, Issue 7, pp. 3990-7, (2013) (PubMed).

Checinska, Soengas: "The gluttonous side of malignant melanoma: basic and clinical implications of macroautophagy." in: Pigment cell & melanoma research, Vol. 24, Issue 6, pp. 1116-32, (2011) (PubMed).

Cherra, Kulich, Uechi, Balasubramani, Mountzouris, Day, Chu: "Regulation of the autophagy protein LC3 by phosphorylation." in: The Journal of cell biology, Vol. 190, Issue 4, pp. 533-9, (2010) (PubMed).

Eby, Rosenbluth, Mays, Marshall, Barton, Sinha, Johnson, Tang, Pietenpol: "ISG20L1 is a p53 family target gene that modulates genotoxic stress-induced autophagy." in: Molecular cancer, Vol. 9, pp. 95, (2010) (PubMed).

Mitroulis, Kourtzelis, Kambas, Rafail, Chrysanthopoulou, Speletas, Ritis: "Regulation of the autophagic machinery in human neutrophils." in: European journal of immunology, Vol. 40, Issue 5, pp. 1461-72, (2010) (PubMed).

English, Chemali, Duron, Rondeau, Laplante, Gingras, Alexander, Leib, Norbury, Lippé, Desjardins: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, (2009) (PubMed).

Details zu LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Andere Bezeichnung: LC3B (MAP1LC3B Produkte)

Synonyme: ATG8F antikoerper, LC3B antikoerper, MAP1A/1BLC3 antikoerper, MAP1LC3B-a antikoerper, Map1lc3 antikoerper, Mpl3 antikoerper, zbs559 antikoerper, 1010001C15Rik antikoerper, Atg8 antikoerper, LC3b antikoerper, zgc:56434 antikoerper, wu:fb60g11 antikoerper, map1lc3b antikoerper, MGC76283 antikoerper, MAP1LC3B antikoerper, microtubule associated protein 1 light chain 3 beta antikoerper, microtubule-associated protein 1 light chain 3 beta antikoerper, microtubule associated protein 1 light chain 3 beta L homeolog antikoerper, MAP1LC3B antikoerper, Map1lc3b antikoerper, map1lc3b antikoerper, map1lc3b.L antikoerper

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Molekulargewicht: 14688

Gen-ID: 81631

NCBI Accession: NP_073729

UniProt: Q9GZQ8

Pathways: Autophagie