Mitochondrial Intermediate Peptidase (MIPEP) (N-Term) Antikörper

Antigen: Mitochondrial Intermediate Peptidase (MIPEP)

Synonyme: MIP, HMIP, 5730405E07Rik, K17N15.9, K17N15_9, DKFZp469L1232

Epitope: N-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN388246

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: MIPEP (N-term)

Gen-ID: 4285

Swiss-Prot: Q99797

Immunogen: This MIPEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187~217 amino acids from the N-terminal region of human MIPEP.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: MIP, HMIP

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MIPEP.

Molekulargewicht: 80612 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting ~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Stammzellen, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of MIPEP (arrow) using rabbit polyclonal MIPEP Antibody (N-term) (ABIN388246). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MIPEP gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MIPEP antibody (N-term) (ABIN388246), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publikationen

Publikationen: Marcondes, Torquato, Assis et al.: "Mitochondrial intermediate peptidase: expression in Escherichia coli and improvement of its enzymatic activity detection with FRET substrates." in: Biochemical and biophysical research communications, Vol. 391, Issue 1, pp. 123-8, 2010 (PubMed).