Insulin-Degrading Enzyme (IDE) (Center) Antikörper

Antigen: Insulin-Degrading Enzyme (IDE)

Synonyme: FLJ35968, INSULYSIN, AA675336, AI507533, 1300012G03Rik, 4833415K22Rik, INSDEGM, IDE, ide, MGC162603

Epitope: Center

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN388241

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: IDE (Center)

Gen-ID: 3416

Swiss-Prot: P14735

Immunogen: This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413~443 amino acids from the Center region of human IDE.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Insulysin, Insulinase, Insulin protease

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human IDE.

Molekulargewicht: 117838 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA1:1,000 Western blotting 1:50~100 Immunohistochemistry ~~ 1:50

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Stammzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of anti-IDE Antibody (Center) (ABIN388241) in A375 cell line lysates (35ug/lane). IDE (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center)(ABIN388241), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publikationen

Publikationen: Radulescu, Poznic, Pavelic: "Complex formation between metabolic enzymes in tumor cells: unfolding the MDR1-IDE paradigm." in: Molecular cancer therapeutics, Vol. 8, Issue 11, pp. 3171, 2009 (PubMed).

Radulescu, Fischer, Stange et al.: "Promoting scientific standards in Germany." in: Science (New York, N.Y.), Vol. 328, Issue 5976, pp. 307, 2010 (PubMed).