SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1) (C-Term) Antikörper

Antigen: SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1)

Synonyme: MGC109561, UBL1, zgc:65934, zgc:85634, hm:zeh0670, wu:fb74c02, wu:fd12c02, SUMO1, ubl1, sumo-1, xsumo1, sumo1-b, xsumo-1, MGC85025, ATSUMO1, F10M23.180, F10M23_180, SMALL UBIQUITIN-LIKE MODIFIER 1, SUM1, SUMO 1, pigSUMO1, sumo1, DKFZp469M0816, GMP1, PIC1, SMT3, DAP-1, OFC10, SENP2, SMT3C, SMT3H3, SUMO-1, Ubl1, SMTP3, Smt3C, SENTRIN, MGC103203

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunfluoreszenz (IF), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN388027

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: SUMO1 (C-term)

Gen-ID: 7341

Swiss-Prot: P63165

Immunogen: This SUMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~93 amino acids from the C-terminal region of human SUMO1.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Small ubiquitin-related modifier 1, SUMO-1, Ubiquitin-like protein SMT3C, SMT3 homolog 3, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein UBL1, GAP modifying protein 1, GMP1, Sentrin, SMT3C, SMT3H3, UBL1

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SUMO1.

Molekulargewicht: 11557 DA

Synonyme: MGC109561, UBL1, zgc:65934, zgc:85634, hm:zeh0670, wu:fb74c02, wu:fd12c02, SUMO1, ubl1, sumo-1, xsumo1, sumo1-b, xsumo-1, MGC85025, ATSUMO1, F10M23.180, F10M23_180, SMALL UBIQUITIN-LIKE MODIFIER 1, SUM1, SUMO 1, pigSUMO1, sumo1, DKFZp469M0816, GMP1, PIC1, SMT3, DAP-1, OFC10, SENP2, SMT3C, SMT3H3, SUMO-1, Ubl1, SMTP3, Smt3C, SENTRIN, MGC103203

Anwendungen

Applikationshinweise: Suggested Dilution: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Apoptose/Nekrose, Zelluläre Signaltransduktion, Proteinmodifikationen, Transkriptionsfaktoren, Signaltransduktion, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

The anti-SUMO1 polyclonal antibody (ABIN388027) is used in Western blot to detect GST-SUMO1 fusion protein.

COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with ABIN388027 SUMO-1 antibody. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with ABIN388034 SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publikationen

Publikationen: Laoong-u-thai, Zhao, Phongdara et al.: "Identifications of SUMO-1 cDNA and its expression patterns in Pacific white shrimp Litopeanaeus vannamei." in: International journal of biological sciences, Vol. 5, Issue 2, pp. 205-14, 2009 (PubMed).