HDAC4 Antikörper (C-Term)

Produktnummer ABIN387951

Produktdetails anti-HDAC4 Antikörper

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Bindungsspezifität: AA 1052-1084, C-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HDAC4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This HDAC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1052-1084 amino acids from the C-terminal region of human HDAC4.

Klon: RB5648

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Andere Bezeichnung: HDAC4 (HDAC4 Produkte)

Synonyme: CG1770 antikoerper, DHDAC4 antikoerper, Dmel\\CG1770 antikoerper, GC1770 antikoerper, HDAC antikoerper, HDAC4a antikoerper, dHDAC4 antikoerper, dmHDA405 antikoerper, hdac4 antikoerper, dana_GLEANR_19966 antikoerper, DanaGF18710 antikoerper, GF18710 antikoerper, HDAC4 antikoerper, AHO3 antikoerper, BDMR antikoerper, HA6116 antikoerper, HD4 antikoerper, HDAC-4 antikoerper, HDAC-A antikoerper, HDACA antikoerper, 4932408F19Rik antikoerper, AI047285 antikoerper, wu:fa96d08 antikoerper, wu:fc56f08 antikoerper, zgc:152701 antikoerper, histone deacetylase 4 antikoerper, hypothetical protein antikoerper, Histone deacetylase 4 antikoerper, GF18710 gene product from transcript GF18710-RC antikoerper, PTRG_03699 antikoerper, PGTG_15512 antikoerper, HDAC4 antikoerper, Dana\GF18710 antikoerper, Hdac4 antikoerper, hdac4 antikoerper

Hintergrund: DNA is wrapped around histone proteins to form nucleosomes and chromatin fiber, a higher-order structure. Chromatin can become alternatively revealed to or concealed from transcription factors. Acetylation of lysine residues induces conformational changes in core histones by destabilizing nucleosomes and allowing transcription factors access to recognition elements in DNA. Deacetylation of histones by histone deacetylases (HDACs) reseals the chromosomal package, leading to a repression of transcription. HDAC4 does not bind DNA directly, but rather through MEF2C and MEF2D. Binding of the N terminus of HDAC4 to MEF2C represses MEF2C transcription activity. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. Experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 result in loss of enzymatic activity associated with HDAC4, indicating regulation of transcription by bridging the enzymatically active NCOR2-HDAC3 complex and select transcription factors. HDAC4 and MITR contain calmodulin-binding domains that overlap with their MEF2 binding domains. Binding of calmodulin to HDAC4 leads to its dissociation from MEF2, relieving MEF2 from the transcriptional repression by HDAC4. Together, HDAC4, MITR, and CABIN1 constitute a family of calcium-sensitive transcriptional repressors of MEF2. In murine studies, HDAC4, which is expressed in prehypertrophic chondrocytes, interacts with and inhibits the activity of Runx2 in mice, a transcription factor necessary for chondrocyte hypertrophy, establishing HDAC4 as a central regulator of chondrocyte hypertrophy and skeletogenesis.

Molekulargewicht: 119040

Gen-ID: 9759

NCBI Accession: NP_006028

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process