His Tag Antikörper

Details zu Produkt Nr. ABIN387901, Anbieter: Anmelden zum Anzeigen
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reaktivität
Tag
616
24
22
14
3
2
Wirt
Kaninchen
435
183
27
23
4
4
3
1
Klonalität (Klon)
Polyklonal ()
Konjugat
Unkonjugiert
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
554
386
167
107
99
77
69
26
13
11
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Poly-HIS peptide were used to produced this antibody.
Sequenz HHHHHH
Klon RB19955
Isotyp Ig
Spezifität Poly-HIS peptide were used to produced this antibody.
Reinigung This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Andere Bezeichnung 6 X His Tag
Substanzklasse Tag
Hintergrund Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Synonyms: Poly-His, Hexa-HIS, Hexa-Histidine
Molekulargewicht 1000 DA
Applikationshinweise WB = 1:1000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Haltbarkeit 6 months
Bilder des Herstellers
Western Blotting (WB) image for anti-His Tag antibody (ABIN387901) Western blot analysis of 6*His Tag Antibody (ABIN387901) in his-tag TBB1 protein (35 ...
Produkt verwendet in: Anderson, Robson, Jiang, Malmirchegini, Fierobe, Lazazzera, Clubb: "Assembly of minicellulosomes on the surface of Bacillus subtilis." in: Applied and environmental microbiology, Vol. 77, Issue 14, pp. 4849-58, 2011 (PubMed).

Allgemeine Veröffentlichungen Hochuli, Döbeli, Schacher: "New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues." in: Journal of chromatography, Vol. 411, pp. 177-84, 1988 (PubMed).

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