V5 Epitope Tag Antikörper

Details zu Produkt Nr. ABIN387850, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
399
18
16
3
3
Wirt
Kaninchen
248
135
34
7
5
4
3
1
1
Klonalität (Klon)
Polyklonal ()
Konjugat
Unkonjugiert
30
27
21
13
12
12
11
11
11
10
10
10
10
9
9
8
8
7
5
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
365
261
136
94
77
71
51
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
Sequenz GKPIPNPLLG LDST
Klon RB0413
Isotyp Ig
Spezifität KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
Reinigung This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Andere Bezeichnung V5-Tag
Substanzklasse Tag
Hintergrund Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). Abgent's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
Molekulargewicht DA
Forschungsgebiet Tags/Labels
Applikationshinweise WB = 1:1000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Haltbarkeit 6 months
Bilder des Herstellers
 image for anti-V5 Epitope Tag antibody (ABIN387850) Lanes: 1:non-transfected cells. 2:V5-tagged empty plasmid. 3:protein A (V5-tagged). 4...
Immunohistochemistry (IHC) image for anti-V5 Epitope Tag antibody (ABIN387850) Western blot analysis of anti-V5 Tag Antibody (ABIN387850) in 12tag protein (35 µg/la...
Allgemeine Veröffentlichungen Dunn, ODowd, Randall: "Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 141-50, 1999 (PubMed).

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