His Tag Antikörper

Details zu Produkt Nr. ABIN387750, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
612
26
15
3
3
Wirt
Maus
410
185
27
23
4
4
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
58
45
36
14
13
11
10
10
9
9
9
9
8
8
8
7
7
7
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
549
389
164
109
78
78
74
26
13
11
9
7
6
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produce this monoclonal antibody.
Sequenz HHHHHH
Klon 6AT18
Isotyp IgG1
Reinigung This antibody is purified through a protein G column, followed by dialysis against PBS.
Substanzklasse Tag
Molekulargewicht 1000 Da
Gen-ID 12406
Forschungsgebiet Tags/Labels
Applikationshinweise Suggested dilutions:
WB = 1:1,000
Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid freeze-thaw cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Haltbarkeit 6 months
Bilder des Herstellers
 image for anti-His Tag antibody (ABIN387750) HIS-tagged recombinant protein was expressed in E. Coli, and used to test the 6xHIS m...
Produkt verwendet in: Kim, Pai, Sato, Person, Nakamura, Macdonald: "Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression." in: PLoS genetics, Vol. 11, Issue 2, pp. e1004992, 2015 (PubMed).

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Bhandari, Lopez-Sanchez, To, Lo, Aznar, Leyme, Gupta, Niesman, Maddox, Garcia-Marcos, Farquhar, Ghosh: "Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration-proliferation dichotomy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 35, pp. E4874-83, 2015 (PubMed).

Shi, Zhang, Guo, Zhang, Hu, Xi, Miao, Li: "131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma." in: Oncology reports, 2015 (PubMed).

Liu, Novick: "Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p." in: The Journal of cell biology, Vol. 207, Issue 1, pp. 59-72, 2014 (PubMed).

Hufnagel, DePas, Chapman: "The disulfide bonding system suppresses CsgD-independent cellulose production in Escherichia coli." in: Journal of bacteriology, Vol. 196, Issue 21, pp. 3690-9, 2014 (PubMed).

Faure, Nagarajan, Hwang, Montgomery, Khan, John, Koulen, Blancaflor, Chapman: "Synthesis of phenoxyacyl-ethanolamides and their effects on fatty acid amide hydrolase activity." in: The Journal of biological chemistry, Vol. 289, Issue 13, pp. 9340-51, 2014 (PubMed).

Malmirchegini, Sjodt, Shnitkind, Sawaya, Rosinski, Newton, Klebba, Clubb: "Novel mechanism of hemin capture by Hbp2, the hemoglobin-binding hemophore from Listeria monocytogenes." in: The Journal of biological chemistry, Vol. 289, Issue 50, pp. 34886-99, 2014 (PubMed).

Wang, Xiang, Zhang, Chen, Zhong, Wang: "Development of a functional antibody by using a green fluorescent protein frame as the template." in: Applied and environmental microbiology, Vol. 80, Issue 14, pp. 4126-37, 2014 (PubMed).

Hsu, Motiwala, Roy, Claus, Mustafa, Plass, Freitas, Ghoshal, Jacob: "Methylation of the PTPRO gene in human hepatocellular carcinoma and identification of VCP as its substrate." in: Journal of cellular biochemistry, Vol. 114, Issue 8, pp. 1810-8, 2013 (PubMed).

Shen, Yuan, Hutagalung, Verma, Kümmel, Wu, Reinisch, McNew, Novick: "The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p." in: The Journal of cell biology, Vol. 202, Issue 3, pp. 509-26, 2013 (PubMed).

Narayanan, Gabrieli, Leung, Elguindy, Glaser, Saju, Sinha, Nakamaru-Ogiso: "Semiquinone and cluster N6 signals in His-tagged proton-translocating NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli." in: The Journal of biological chemistry, Vol. 288, Issue 20, pp. 14310-9, 2013 (PubMed).

Feltcher, Gibbons, Ligon, Braunstein: "Protein export by the mycobacterial SecA2 system is determined by the preprotein mature domain." in: Journal of bacteriology, Vol. 195, Issue 4, pp. 672-81, 2013 (PubMed).

Anderson, Miller, Fierobe, Clubb: "Recombinant Bacillus subtilis that grows on untreated plant biomass." in: Applied and environmental microbiology, Vol. 79, Issue 3, pp. 867-76, 2013 (PubMed).

Rytinki, Kaikkonen, Sutinen, Palvimo: "Analysis of androgen receptor SUMOylation." in: Methods in molecular biology (Clifton, N.J.), Vol. 776, pp. 183-97, 2011 (PubMed).

Ye, Selby, Ozturk, Annayev, Sancar: "Biochemical analysis of the canonical model for the mammalian circadian clock." in: The Journal of biological chemistry, Vol. 286, Issue 29, pp. 25891-902, 2011 (PubMed).

Yilmaz, Sancar, Kemp: "Multiple ATR-Chk1 pathway proteins preferentially associate with checkpoint-inducing DNA substrates." in: PLoS ONE, Vol. 6, Issue 7, pp. e22986, 2011 (PubMed).

Terasawa, Toya, Motegi, Mana, Nakamura, Sugimoto: "Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 15, Issue 11, pp. 1145-57, 2010 (PubMed).

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