DYKDDDDK Tag Antikörper (FITC)

Details zu Produkt Nr. ABIN387724, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
232
119
65
18
18
10
9
7
4
3
2
1
1
1
Wirt
Maus
191
130
34
34
17
Klonalität (Klon)
Monoklonal ()
Konjugat
FITC
40
21
19
10
10
10
9
9
8
7
7
7
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF)
331
131
124
105
86
59
44
14
11
8
7
7
4
4
4
4
3
3
3
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke THE™
Immunogen A synthetic peptide DYKDDDDK coupled - KLH
Sequenz DYKDDDDK
Klon 5A8E5
Isotyp IgG
Spezifität THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.
Produktmerkmale This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Reinigung Protein A affinity column
Substanzklasse Tag
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/mL
Flow cytometry: 0.5-2 µg for 1 x 10^6 cells
Other applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Konzentration 0.5 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-DYKDDDDK Tag antibody (FITC) (ABIN387724) Flow cytometric analysis of transfected HEK-293 cell expressing HA-tagged protein usi...
Produkt verwendet in: Pumroy, Nardozzi, Hart, Root, Cingolani: "Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin ?5." in: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 2022-31, 2012 (PubMed).

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