BH3 Interacting Domain Death Agonist (BID) Antikörper

Antigen: BH3 Interacting Domain Death Agonist (BID)

Synonyme: FP497, MGC15319, MGC42355, AI875481, AU022477, 2700049M22Rik, BID, MGC140420, bid, si:ch211-238n5.6, xbid, fp497, DKFZp469E066

Klonalität: Monoklonal (SB50a)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN377017

Produktbeschreibung

Weitere Bezeichnung: Bid

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: SB50a

Spezifität: Human Bid Members of the Bcl-2 family are pro- or antiapoptotic proteins that process one or several of the Bcl-2 homology (BH) domains. Bid is a proapoptotic, BH3-domain-only member or BH3-only protein (BOP) of the Bcl-2 family. Bid is localized in the cytosolic fraction of living cells as an inactive precursor. Activation of Bid occurs upon its cleavage by caspase-8 in the Fas and TNF- alpha apoptotic signaling pathways. Recent studies have suggested that Bid is also cleaved and activated by granzyme B, calpain and lysosomal extracts. Cleaved Bid translocates to mitochondria where it induces the release of cytochrome c from the intermembrane space of the mitochondria. The release of cytochrome c initiates apoptosis by activating downstream caspases through binding to Apaf-1. Bid has also been shown to interact with with Bax, another Bcl-2 family protein, to trigger a conformational change in Bax leading to the dimerization and integration of Bax into the outer mitochondrial membrane and activating the release of cytochrome c.

Anwendungen

Applikationshinweise: To insure lot-to-lot consistency, each batch of product is tested by western blotting to conform with the characteristics of a standard reference reagent.

Buffer: The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. Reagents are stable for the period shown on the label

Lagerung: Store at 2-8°C

Forschungsgebiet: Krebs, Apoptose/Nekrose

Beschränkungen: Nur für Forschungszwecke einsetzbar