Biotin Antikörper
| Antigen |
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| Klonalität |
Monoklonal (SB58c) |
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Wirt
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Applikation
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Western Blot (WB) (83), ELISA (63), Durchflusszytometrie (FACS) (57), Immunfluoreszenz (IF) (44), Immunpräzipitation (IP) (37), Enzyme Immunoassay (EIA) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (30), Immunhistochemie (IHC) (24), Immunhistochemie (Paraffinschnitte) (IHC (p)) (14), Dot Blot (Dot) (8), Immunodiffusion (ID) (6), Immunzytochemie (ICC) (3), Immunelektrophorese (IEP) (1), Radioimmunoassay (RIA) (1)
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| Produktnummer |
ABIN376975 |
| Menge |
0.50 mg |
| Preis |
134,91 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Immunogen
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KLH1139-biotin
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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SB58c
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Spezifität
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Biotin Anti-Biotin can be used for indirect immuno-staining when working with a biotinylated primary antibody. It can be used for simultaneous staining of several biotinylated antibodies as one of the parameters in certain assay platforms (such as Luminex). It is an alternative technology to magnify the signal and to reduce background caused by avidin or its derivatives.
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Applikationshinweise
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To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or PCFIA for conformance to characteristics of a standard reference reagent. Purified antibody conjugate: <= 1 µg/10 6 cells, Purified antibody HRP conjugate: 1 µg/mL 1:4,000-1:8,000.
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Buffer
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The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added . Store at 2-8°C. The horseradish peroxidase (HRP) conjugate is supplie
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Lagerung
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Store at 2-8°C
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Forschungsgebiet
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Signaltransduktion, Metabolismus, Epitop-Tags, Tags/Labels
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Biotin", Typ "Antikörper" finden
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Wirte
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Ziege (105), Kaninchen (30), Maus (29), Schaf (3)
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Reaktivitäten
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Alle Spezies (1), Human (1)
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Applikationen
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Western Blot (WB) (83), ELISA (63), Durchflusszytometrie (FACS) (57), Immunfluoreszenz (IF) (44), Immunpräzipitation (IP) (37), Enzyme Immunoassay (EIA) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (30), Immunhistochemie (IHC) (24), Immunhistochemie (Paraffinschnitte) (IHC (p)) (14), Dot Blot (Dot) (8), Immunodiffusion (ID) (6), Immunzytochemie (ICC) (3), Immunelektrophorese (IEP) (1), Radioimmunoassay (RIA) (1)
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Konjugate
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FITC (21), HRP (20), Alkaline Phosphatase (AP) (10), Texas Red (TR) (10), Cy2 (5), Cy5.5 (4), PE (4), Cy3.5 (3), Biotin (2), Colloidal Gold (15 OD) (2), Cy5 (2), Rhodamine (2), TRITC (2), 488-NHS Ester (1), DTAF (1), Peroxidase (POD) (1)
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Alternativen 
