DDX5 Antikörper (C-Term)

Produktnummer ABIN374414

Produktdetails anti-DDX5 Antikörper

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DDX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-PMIGYPMPTG YSQ

Spezifität: This antibody detects DEAD Box Protein 5 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_004387.1. Genename: DDX5

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.11-0.3 μg/mL. Approx 70 kDa band observed in human epidermoidcarcinoma A431 lysates (calculated MW of 69.0 kDa according to NP_004387). Immunofluorescence: A customer has reported successful staining at a dilution of 1: 100 onHT-1080 cells with a Rhodamine conjugated secondary.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Details zu DDX5

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Andere Bezeichnung: DDX5 (DDX5 Produkte)

Synonyme: G17P1 antikoerper, HLR1 antikoerper, HUMP68 antikoerper, p68 antikoerper, 2600009A06Rik antikoerper, Hlr1 antikoerper, wu:fa56a07 antikoerper, wu:fb11e01 antikoerper, wu:fb16c10 antikoerper, wu:fb53b05 antikoerper, ddx5 antikoerper, DEAD-box helicase 5 antikoerper, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 antikoerper, DEAD (Asp-Glu-Ala-Asp) box helicase 5 antikoerper, DEAD-box helicase 5 L homeolog antikoerper, DDX5 antikoerper, Ddx5 antikoerper, ddx5 antikoerper, ddx5.L antikoerper

Hintergrund: DDX belongs to the family of DEAD box proteins, characterized by the conserved motif Asp/Glu/Ala/Asp, which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.Synonyms: DDX-5, DEAD box protein 5, G17P1, HELR, HLR1, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68

Molekulargewicht: 69.1 kDa according to NP_004387

Gen-ID: 1655

NCBI Accession: NP_004387

UniProt: P17844

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus