Aurora A Antikörper (C-Term)

Produktnummer ABIN374071

Produktdetails anti-Aurora A Antikörper

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Aurora A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: C-QNKESASKQS

Spezifität: This antibody detects Aurora kinase A / AURKA at C-term.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_003591.2.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection limit dilution 1: 128000. Western blot: 0.1-0.3 μg/mL. Approx 45 kDa band observed in lysates of cell line HeLa andJurkat lysate (calculated MW of 45.5 kDa according to NP_003591.2). In transfected HEK293transiently expressing AURKA a band of approx.45 kDa is observed. This band is notobserved in the non-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Andere Bezeichnung: Aurora Kinase A (AURKA Produkte)

Hintergrund: Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6

Gen-ID: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Zellzyklus, Asymmetric Protein Localization