GRIN2B Antikörper (pTyr1336)

Produktnummer ABIN372686

Produktdetails anti-GRIN2B Antikörper

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Bindungsspezifität: pTyr1336

Reaktivität: Human, Ratte, Maus, Non-Human Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRIN2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody is specific for ~180k NMDAR NR2B subunit protein phosphorylated at Tyr1336. Immunolabeling of the NMDAR NR2B subunit band is blocked by lambda-phosphatase treatment.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1336 of the NR2B subunit of the rat NMDA receptor.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Referenzen für anti-GRIN2B Antikörper (ABIN372686)

Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, (2011) (PubMed).

Details zu GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Andere Bezeichnung: NMDAR2B (GRIN2B Produkte)

Synonyme: NMDAR2B antikoerper, GluN2B antikoerper, MRD6 antikoerper, NR2B antikoerper, hNR3 antikoerper, nmdar2b antikoerper, nr2b antikoerper, AW490526 antikoerper, Nmdar2b antikoerper, glutamate ionotropic receptor NMDA type subunit 2B antikoerper, glutamate receptor, ionotropic, N-methyl D-aspartate 2B L homeolog antikoerper, glutamate receptor, ionotropic, NMDA2B (epsilon 2) antikoerper, GRIN2B antikoerper, Grin2b antikoerper, grin2b.L antikoerper

Hintergrund: The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1336 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003).Synonyms: GRIN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA Receptor 2B

Gen-ID: 2904

NCBI Accession: NP_000825

UniProt: Q13224

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity