CAMK2A Antikörper (pThr286)

Produktnummer ABIN372594

Produktdetails anti-CAMK2A Antikörper

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Bindungsspezifität: pThr286

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAMK2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Specific for the ~50k Alpha-CaM Kinase II and the ~60k Beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the lambda-phosphatase treatment.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Mouse and Xenopus.
Species reactivity (tested):Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr286 found in rat brain CaM Kinase II.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Referenzen für anti-CAMK2A Antikörper (ABIN372594)

Magupalli, Mochida, Yan, Jiang, Westenbroek, Nairn, Scheuer, Catterall: "Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2.1 calcium channels." in: The Journal of biological chemistry, Vol. 288, Issue 7, pp. 4637-48, (2013) (PubMed).

Kothmann, Trexler, Whitaker, Li, Massey, OBrien: "Nonsynaptic NMDA receptors mediate activity-dependent plasticity of gap junctional coupling in the AII amacrine cell network." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 20, pp. 6747-59, (2012) (PubMed).

Coultrap, Buard, Kulbe, DellAcqua, Bayer: "CaMKII autonomy is substrate-dependent and further stimulated by Ca2+/calmodulin." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17930-7, (2010) (PubMed).

Vest, OLeary, Coultrap, Kindy, Bayer: "Effective post-insult neuroprotection by a novel Ca(2+)/ calmodulin-dependent protein kinase II (CaMKII) inhibitor." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20675-82, (2010) (PubMed).

Sun, Milovanovic, Zhao, Wolf: "Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 16, pp. 4216-30, (2008) (PubMed).

Davies, Goebel-Goody, Coultrap, Browning: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, (2008) (PubMed).

Vest, Davies, OLeary, Port, Bayer: "Dual mechanism of a natural CaMKII inhibitor." in: Molecular biology of the cell, Vol. 18, Issue 12, pp. 5024-33, (2007) (PubMed).

Details zu CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Andere Bezeichnung: CAMK2A (CAMK2A Produkte)

Synonyme: camk2 antikoerper, camk2b antikoerper, zgc:112538 antikoerper, zgc:123320 antikoerper, CAMKA antikoerper, CaMKII antikoerper, R74975 antikoerper, mKIAA0968 antikoerper, PK2CDD antikoerper, PKCCD antikoerper, calcium/calmodulin dependent protein kinase II alpha antikoerper, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog antikoerper, calcium/calmodulin-dependent protein kinase II alpha antikoerper, CAMK2A antikoerper, camk2a.S antikoerper, camk2a antikoerper, Camk2a antikoerper

Hintergrund: Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998, Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003, Soderling, 2000, Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003, Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/calmodulin-dependent protein kinase type II, KIAA0968

Gen-ID: 815

NCBI Accession: NP_741960

UniProt: Q9UQM7

Pathways: WNT Signalweg, Interferon-gamma Pathway, Myometrial Relaxation and Contraction